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Korean J Crit Care Med > Volume 13(1); 1998 > Article
Korean Journal of Critical Care Medicine 1998;13(1): 25-32.
백서의 적출된 기도에서 Lipopolysaccharide 에 의한 반응과 상피세포 제거에 의한 영향
신효철, 김윤희, 허동식, 윤석화, 신용섭, 최세진
충남대학교 의과대학 마취과학교실
The Effects of Lipopolysaccharide on the Reactivity of Isolated Rat Trachea with or without Epithelium
Hyo Chul Shin, Yoon Hee Kim, Dong Sik Hur, Seok Hwa Yoon, Yong Sup Shin, Sae Jin Choi
Department of Anesthesiology, Chungnam National University, College of Medicine, Taejon, Korea.
BACKGOUND: Gram negative bacterial lipopolysaccharide (LPS) induces increase in the production of nitric oxide (NO), or a related substance derived from L-arginine in the animal tissue. Recent evidence indicates that airway epithelium may secrete NO or a related compound. It has multiple regulatory roles in the airways. In vitro, the effects of lipopolysaccharide (LPS) on the reactivity of rat' tracheal wall with or without epithelium were examined. METHODS: Tracheas were removed from Sprague Dawley rats. Preparations were mounted for isometric recording in 20ml organ baths at 37degrees C containing Tis-buffered Tyrode solution continuously gassed with 100% O2. Tensions were measured with force displacement transducers and responses were recorded on a polygraph. Cummulative concentration-response curves were constructed for acetylcholine (Ach) in the tracheal strips with or without preincubation of Escherichia coli LPS (100 mcg/ml, 5hrs). And then effects of NO synthase inhibitors and removal of epithelium were examined. RESULTS: In isolated perfused tracheas preincubated by LPS, both removed epithelium and intact epithelium of rat tracheal rings showed decreased Ach-induced contraction. In intact epithelium group, 10 (-5)M L-NAME (N-nitro-L-arginine methyl ester), 10 (-5)M L-arginine or dexamethasone pretreatment was restored in Ach-induced contraction response. But in the removed epithelium group, Ach-induced contraction was potentiated by L-arginine pretreatment and was not restored by the pretreatment of L-NAME and dexamethasone. CONCLUSIONS: The results suggest that nitric oxide synthase is induced by endotoxin in the tracheal epithelium, resulting in inhibition of the contractile response.
Key Words: Animals; rats; Airway; trachea; Immune Response; endotoxin
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